The correct use of the microscope

       The electron microscope   Electron microscopy (EM) is a technique for obtaining high resolution images of biological and non-biological specimens. It is used in biomedical research to investigate the detailed structure of tissues, cells, organelles and macromolecular complexes. The high resolution of EM images results from the use of electrons (which have very short wavelengths) as the source of illuminating radiation. Electron microscopy is used in conjunction with a variety of ancillary techniques (e.g. thin sectioning, immuno-labeling, negative staining) to answer specific questions. EM images provide key information on the structural basis of cell function and of cell disease.   There are two main types of electron microscope – the transmission EM (TEM) and the scanning EM (SEM). The transmission electron microscope is used to view thin specimens (tissue sections, molecules, etc) through which electrons can pass generatin...

  Compound light microscope        A compound microscope is a high power (high magnification) microscope that uses a compound lens system. A compound microscope has multiple lenses: the   objective lens   (typically 4x, 10x, 40x or 100x) is compounded (multiplied) by the   eyepiece lens   (typically 10x) to obtain a high magnification of 40x, 100x, 400x and 1000x. Higher magnification is achieved by using two lenses rather than just a single magnifying lens. While the eyepieces and the objective lenses create high magnification, a condenser beneath the stage focuses the light directly into the sample.  

   The simple microscope   A simple microscope is essentially a magnifying glass made of a single convex lens with a short focal length, which magnifies the object through angular magnification, thus producing an erect virtual image of the object near the lens.